Composite

Part:BBa_K2598051:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-02)


ho1+pcyA+CI+K1F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 811
    Illegal NheI site found at 1543
    Illegal NheI site found at 1566
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcyA and ho1 are reversed to avoid leakage.


Source

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)